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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Does ligninolytic enzyme activity depend on phenolics content during the litter decomposition?
APPLOVÁ, Markéta
The aim of the study was to determine the influence of phenolics content and inoculation with soil extract on microbial respiration, on the phenoloxidase (PhOx), peroxidase (PerOx) and newly Mn-peroxidase (MnP) activity in two dominating litter samples (Calamagrostis villosa and Picea abies) differing in phenolics content from Plešné and Čertovo lake watersheds. At PhOx and PerOx activity, the dependence on incubation temperature with L-DOPA was estimated. PhOx and MnP activities significantly increased with higher content of hardly decomposable phenolics, but decreased with water extractable phenolics content. Inoculation with soil extract had no influence on microbial respiration, enzyme activity, nor on decomposition of phenolics. Microbial respiration was significantly higher at 10°C, but average enzyme activity was comparable at 0 and 10°C. PhOx activities had temperature optimum higher than 22°C, while PerOx activities had temperature optimum at 0 - 15°C.
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Acitivity of phenoloxidases and peroxidases in the litter of spruce forest in Plešné and Čertovo lake watersheds
 APPLOVÁ, Markéta
The aim of the study was to determine the activity of phenoloxidases and peroxidases in three dominating litter samples (Vaccinium myrtillus, Calamagrostis villosa and Picea abies) from Plešné and Čertovo lake watersheds. High activity of lignin-degrading enzymes was detected in litter incubated at 0°C. Activity of enzymes was in general higher at 10°C but the difference was not significant compared to 0°C. In Plešné Lake watershed high amount of phenolic compounds negatively influenced the activity of enzymes compared to Čertovo Lake watershed, where the effect of phenolic compounds was opposite. The highest activities of the enzymes were found in the Calamagrostis villosa litter from Plešné Lake watershed and in the litter of spruce needles from Čertovo lake watershed.
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